Dhuley J N, Naik S R
Department of Pharmacology & Toxicology, Hindustan Antibiotics Ltd, Pimpri, Pune, India.
Indian J Exp Biol. 1996 Oct;34(10):1010-4.
Effect of 6-MFA (sixth mycelial fraction of acetone), an interferon inducer obtained from fungus A. ochraceus on hepatic mixed function oxidase system (MFO) of rat has been investigated. Treatment with 6-MFA, 100 mg/kg/day, ip for 1-5 days to adult rats inhibited significantly the different indices of MFO system, viz. hepatic cytochrome P-450, cytochrome b5 content, cytochrome c reductase, aminopyrine-N-demethylase and acetanilide hydroxylase activities. Similar treatment for 3 days in young growing rats significantly inhibited MFO system's components except acetanilide hydroxylase activity which showed marked elevation. These effects seem to be specific as in vitro experiments suggested that 6-MFA does not compete with subsdtrates nor it acts as a sponge reacting with the end product to give false inhibitory effect. It is concluded from the present study that 6-MFA like other interferon inducers depresses MFO system in rats. Its possible clinical implications are discussed.
研究了从棕曲霉中获得的干扰素诱导剂6-MFA（丙酮提取的第六菌丝体组分）对大鼠肝脏混合功能氧化酶系统（MFO）的影响。以100mg/kg/天的剂量腹腔注射6-MFA，对成年大鼠连续处理1 - 5天，显著抑制了MFO系统的不同指标，即肝细胞色素P-450、细胞色素b5含量、细胞色素c还原酶、氨基比林-N-脱甲基酶和乙酰苯胺羟化酶活性。对幼年生长大鼠进行类似的3天处理，除乙酰苯胺羟化酶活性显著升高外，显著抑制了MFO系统的组分。这些作用似乎具有特异性，因为体外实验表明，6-MFA既不与底物竞争，也不作为海绵与终产物反应产生假抑制作用。从本研究得出结论，6-MFA与其他干扰素诱导剂一样，会抑制大鼠的MFO系统。并讨论了其可能的临床意义。
