Boguta M, Czerska K, Zoładek T
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Gene. 1997 Feb 7;185(2):291-6. doi: 10.1016/s0378-1119(96)00669-5.
Mutation in the MAF1 gene was identified in a screen for decreased efficiency of tRNA suppressor SUP11 in the yeast Saccharomyces cerevisiae (Sc). maf1-1 mutation exerts a dual phenotypic effect: antisuppression and temperature sensitive (ts) respiratory growth. MAF1, cloned by complementation of the ts phenotype of maf1-1, also alleviates the antisuppressor effect. The coding sequence of MAF1 is interrupted by an intron of 80 bp. The putative gene product, Maf1p, is a hydrophilic protein of 395 amino acids (aa) not showing significant similarity to known proteins which indicates that MAF1 encodes a novel protein. Maf1p may play a role in the tRNA biosynthetic pathway since a fragment of the RPO31/RPC160 gene encoding the largest subunit of RNA polymerase III was cloned as a multicopy suppressor of mafl-1.
在对酿酒酵母（Sc）中tRNA抑制因子SUP11效率降低的筛选中，鉴定出MAF1基因发生了突变。maf1-1突变具有双重表型效应：反抑制和温度敏感（ts）呼吸生长。通过对maf1-1的ts表型进行互补克隆得到的MAF1，也能减轻反抑制效应。MAF1的编码序列被一个80 bp的内含子打断。推测的基因产物Maf1p是一种由395个氨基酸（aa）组成的亲水性蛋白质，与已知蛋白质没有明显的相似性，这表明MAF1编码一种新型蛋白质。由于编码RNA聚合酶III最大亚基的RPO31/RPC160基因的一个片段被克隆为mafl-1的多拷贝抑制因子，因此Maf1p可能在tRNA生物合成途径中发挥作用。
