Shinkai T, Zhang L, Mathias S A, Roth G S
Molecular Physiology and Genetics Section, NIA/NIH, Baltimore, Maryland 21224, USA.
J Neurosci Res. 1997 Feb 15;47(4):393-9.
We examined D2-dopamine receptor containing neurons in cultures of neonatal rat striatum for apoptosis following dopamine treatment. Exposure to cultures to micromolar concentrations of dopamine resulted in 60-70% killing of D2-dopamine receptor neurons within 24 hr. We also utilized a double labeling procedure to determine that treatment with dopamine induced apoptosis in D2-dopamine receptor containing neurons. These results suggest that loss of D2-dopamine receptor containing neurons during aging could be due to an apoptotic effect of dopamine.
我们研究了新生大鼠纹状体培养物中含D2 - 多巴胺受体的神经元在多巴胺处理后的凋亡情况。将培养物暴露于微摩尔浓度的多巴胺中，在24小时内导致60 - 70%的D2 - 多巴胺受体神经元死亡。我们还采用了双重标记程序来确定多巴胺处理可诱导含D2 - 多巴胺受体的神经元发生凋亡。这些结果表明，衰老过程中含D2 - 多巴胺受体的神经元丧失可能是由于多巴胺的凋亡作用。
