Willén H
Department of Clinical Pathology and Cytology, University Hospital, Lund, Sweden.
Acta Orthop Scand Suppl. 1997 Feb;273:47-53. doi: 10.1080/17453674.1997.11744702.
Fine needle aspiration (FNA) is an important part of the preoperative diagnosis in bone tumors. The diagnosis must be based on clinical, radiologic and morphologic correlation. In palpable lesions, FNA is performed on the most accessible part of the tumor. Deep-seated and/or non-palpable lesions need radiologic guidance. Material from the FNA can be used for additional examinations, i.e. electron microscopy, immunocytochemistry, cytochemistry, DNA-ploidy analysis, chromosomal analysis and molecular genetics. Those examinations are of particular importance in the primary and differential diagnosis of Ewing sarcoma, osteosarcoma and chondrosarcoma. The majority of tumors in FNA aspirates can be classified as primary (benign or malignant) and metastatic tumors. Cellularity, pleomorphism, chromatin pattern, nucleolar structure, mitotic figures and necrosis are parameters of malignancy.
细针穿刺抽吸活检（FNA）是骨肿瘤术前诊断的重要组成部分。诊断必须基于临床、放射学和形态学的相互关联。对于可触及的病变，在肿瘤最易接近的部位进行FNA。深部和/或不可触及的病变则需要放射学引导。FNA获取的材料可用于其他检查，如电子显微镜检查、免疫细胞化学、细胞化学、DNA倍体分析、染色体分析和分子遗传学。这些检查在尤因肉瘤、骨肉瘤和软骨肉瘤的原发性和鉴别诊断中尤为重要。FNA抽吸物中的大多数肿瘤可分为原发性（良性或恶性）肿瘤和转移性肿瘤。细胞数量、多形性、染色质模式、核仁结构、有丝分裂象和坏死是恶性肿瘤的参数。
