Croppo G P, Visvesvara G S, Leitch G J, Wallace S, De Groote M A
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.
J Parasitol. 1997 Feb;83(1):66-9.
Microsporidia spores, identified as Encephalitozoon cuniculi (CDC: V282), were isolated from the urine of a patient with acquired immunodeficiency syndrome and disseminated microsporidiosis, established in continuous culture on monkey kidney cells (E6), and antiserum was produced in rabbits. Immunoblot studies that used the patient serum and the rabbit sera against CDC:V282, Encephalitozoon hellem (CDC:0291:V213), and Encephalitozoon intestinalis (CDC:V297) revealed that CDC:V282 and the rabbit isolate of E. cuniculi (ECLD) reacted intensely with the patient's serum and the rabbit anti-CDC:V282, producing a number of bands ranging from 200 to 15 kDa. By contrast, the heterologous antigens (CDC:0291:V213 and CDCV297) reacted minimally. Both CDC:V282 and ECLD isolates of E. cuniculi reacted minimally with the rabbit anti-E. hellem and the rabbit anti-E. intestinalis sera. In the immunofluorescence test, performed on the lung biopsy section of the patient, the rabbit anti-CDC:V282 serum reacted extensively with the spores in the tissue section and produced bright apple green fluorescence. These studies demonstrated that the human (CDC:282) and the rabbit (ECLD) isolates of E. cuniculi were similar in their antigenic profiles but differed considerably from E. hellem and E. intestinalis, and that the patient's serum reacted specifically, strongly, and with equal intensity, with the 2 isolates of E. cuniculi.
从一名获得性免疫缺陷综合征合并播散性微孢子虫病患者的尿液中分离出被鉴定为兔脑炎微孢子虫（疾病控制与预防中心：V282）的微孢子虫孢子，在猴肾细胞（E6）上进行连续培养，并在兔体内产生抗血清。使用患者血清以及针对疾病控制与预防中心：V282、海伦脑炎微孢子虫（疾病控制与预防中心：0291:V213）和肠脑炎微孢子虫（疾病控制与预防中心：V297）的兔血清进行的免疫印迹研究表明，疾病控制与预防中心：V282和兔源兔脑炎微孢子虫分离株（ECLD）与患者血清以及兔抗疾病控制与预防中心：V282强烈反应，产生一系列分子量范围从200到15 kDa的条带。相比之下，异源抗原（疾病控制与预防中心：0291:V213和疾病控制与预防中心V297）反应微弱。兔脑炎微孢子虫的疾病控制与预防中心：V282和ECLD分离株与兔抗海伦脑炎微孢子虫血清和兔抗肠脑炎微孢子虫血清反应微弱。在对患者的肺活检切片进行的免疫荧光试验中，兔抗疾病控制与预防中心：V282血清与组织切片中的孢子广泛反应，并产生明亮的苹果绿色荧光。这些研究表明，人源（疾病控制与预防中心：282）和兔源（ECLD）兔脑炎微孢子虫分离株在抗原谱方面相似，但与海伦脑炎微孢子虫和肠脑炎微孢子虫有很大差异，并且患者血清与兔脑炎微孢子虫的2个分离株特异性、强烈且以相同强度反应。
