Neuritogenesis, not receptor expression, of NG108-15 cells can be modulated by monosialoganglioside GM1.

In this study, involvement of gangliosides in neurite outgrowth and receptor expression of the neuroblastoma X glioma hybrid NG108-15 cloned cells was investigated. Monosialoganglioside GM1 (100 microM) and disialoganglioside GD1a (100 microM) were applied to the culture medium at different concentrations of fetal bovine serum, 1-10%, with or without addition of dibutyryl adenosine 3',5'-cyclic monophosphate (500 microM). In some experiments, 5 mg/ml of cholera toxin B was added to the media to block endogenous GM1. The results indicated that GM1 had an influence on cell proliferation and neuritogenesis but did not induce muscarinic receptor expression of NG108-15 cells.