Ohsaka Y, Yoshimura N, Hamashima T, Yura H, Yasui H, Shiho O, Oka T
Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Eur Surg Res. 1997;29(2):107-15. doi: 10.1159/000129514.
In order to elucidate the immunological characteristics of rat liver transplantation, graft-infiltrating cells (GIC) isolated from rat hepatic allografts were analyzed phenotypically and functionally. GIC from long-surviving recipients (Brown Norway livers into Lewis hosts) and acutely rejecting recipients (DA livers into Lewis hosts) were compared. The relative proportions of all T cells and activated T cells determined by flow cytometry were significantly higher in acutely rejecting Lewis recipients than in long-surviving recipients on day 6 after grafting. Phenotypic kinesis of GIC on days 6, 14, and 45 after transplantation from long-surviving Lewis hosts was analyzed. Each proportion of all T cells, OX8-positive cells (cytotoxic T and natural killer cells), and OX39-positive cells (IL-2 receptor), was greatest on day 6 and decreased by day 45. Cytotoxic activity of GIC toward donor lymphocytes on day 6 was greater in acutely rejecting versus long-surviving recipients. These results demonstrate that an immunosuppressive mechanism is already present on day 6 posttransplantation, and that infiltration or activation of cytotoxic T cells is inhibited in the long-surviving rat hepatic allografts.
为阐明大鼠肝移植的免疫特性，对从大鼠肝同种异体移植物中分离出的移植物浸润细胞（GIC）进行了表型和功能分析。比较了长期存活受体（布朗挪威大鼠肝脏移植到刘易斯大鼠宿主）和急性排斥受体（DA大鼠肝脏移植到刘易斯大鼠宿主）的GIC。移植后第6天，通过流式细胞术测定的急性排斥刘易斯受体中所有T细胞和活化T细胞的相对比例显著高于长期存活受体。分析了长期存活的刘易斯宿主移植后第6天、14天和45天GIC的表型动态变化。所有T细胞、OX8阳性细胞（细胞毒性T细胞和自然杀伤细胞）和OX39阳性细胞（IL-2受体）的比例在第6天最高，到第45天下降。与长期存活受体相比，急性排斥受体中GIC在第6天对供体淋巴细胞的细胞毒性活性更高。这些结果表明，移植后第6天已经存在免疫抑制机制，并且在长期存活的大鼠肝同种异体移植物中，细胞毒性T细胞的浸润或活化受到抑制。
