Trondsen E, Rosseland A R, Clausen O P, Bakka A, Engh E, Rødland E A, Aasen A O
Institute for Surgical Research, National Hospital, University of Oslo, Norway.
Eur Surg Res. 1997;29(2):150-8. doi: 10.1159/000129519.
To study consequences of cystic duct occlusion, 121 NMRI mice were operated: 40 had cystic duct ligature (L group), 41 had cystic duct ligature and evacuation of bile from the gallbladder (LE group), and 40 were sham-operated (S group). Ten mice from each group were sacrificed at 1 week, 1, 3 and 6 months, respectively. All but 2 mice showed signs of wellbeing during the observation time, and weight gain was the same in the three groups. Distended gallbladder occurred in 14 animals, 7 each in the L and the LE group, more frequent after 3 and 6 months. In the remainder mice the gallbladders were smaller than at the time of operation (p < 0.0001). Fibrosis and adhesions around the gallbladder were frequent in the L and the LE groups, and more pronounced in the latter (p = 0.0001). At microscopy there were signs of inflammation in the gallbladder wall in 16 cases in the L group, 28 in the LE group and 2 in the S group, and the degree of inflammation was more pronounced in the LE group than in the L group (p = 0.002). Eleven mice in the LE group had empyema, and slight intralumininal inflammation was more frequent in the LE group than in the L group (p < 0.0001). In conclusion, despite a high incidence of microscopic inflammation the study gave no indication that occlusion of the cystic duct did serious harm to the mice in this series.
为研究胆囊管阻塞的后果，对121只NMRI小鼠进行了手术：40只进行胆囊管结扎（L组），41只进行胆囊管结扎并从胆囊排空胆汁（LE组），40只进行假手术（S组）。每组分别在1周、1个月、3个月和6个月时处死10只小鼠。在观察期间，除2只小鼠外，所有小鼠均表现良好，三组体重增加相同。14只动物出现胆囊扩张，L组和LE组各7只，3个月和6个月后更为常见。其余小鼠的胆囊比手术时小（p < 0.0001）。L组和LE组胆囊周围纤维化和粘连常见，且LE组更明显（p = 0.0001）。显微镜检查显示，L组16例、LE组28例和S组2例胆囊壁有炎症迹象，且LE组炎症程度比L组更明显（p = 0.002）。LE组11只小鼠发生积脓，且LE组管腔内轻微炎症比L组更常见（p < 0.0001）。总之，尽管显微镜下炎症发生率较高，但该研究未表明该系列中胆囊管阻塞对小鼠造成严重损害。
