Litchfield T M, Whiteley S J, Yee K T, Tyers P, Usherwood E J, Nash A A, Lund R D
Department of Pathology, Institute of Ophthalmology, London, UK.
J Neuroimmunol. 1997 Mar;73(1-2):135-44. doi: 10.1016/s0165-5728(96)00192-0.
We have looked at both donor and host MHC expression in a neural xenograft rejection paradigm. Grafts of either mouse corpus callosum or an SV40 large T transformed astrocytic cell line were placed in the mid-brain of neonatal rats. Three weeks later graft rejection was induced by the application of a skin graft of the same donor origin. MHC expression in the neural graft and the host brain was examined histologically four and ten days after the animals had received a skin graft. Donor MHC expression was detected in the corpus callosal grafts at both time points and preceded host MHC expression and the lymphocytic infiltrate. The grafts of the transformed cell line could not be induced to express MHC antigens under the experimental protocol used nor were they rejected. The migratory patterns of the transformed cells were compared to the well characterised migration patterns of astrocytes from the corpus callosal grafts.
我们已在神经异种移植排斥范例中研究了供体和宿主的主要组织相容性复合体（MHC）表达情况。将小鼠胼胝体移植物或SV40大T抗原转化的星形胶质细胞系移植到新生大鼠的中脑。三周后，通过移植来自相同供体的皮肤诱导移植物排斥反应。在动物接受皮肤移植后的第4天和第10天，对神经移植物和宿主脑中的MHC表达进行组织学检查。在两个时间点均在胼胝体移植物中检测到供体MHC表达，且早于宿主MHC表达和淋巴细胞浸润。在所采用的实验方案下，转化细胞系的移植物无法被诱导表达MHC抗原，也未被排斥。将转化细胞的迁移模式与胼胝体移植物中特征明确的星形胶质细胞迁移模式进行了比较。
