Bomans J, Lambert C A, Scarpa B, Nusgens B, Legros W, Lapière C M
Laboratoire de Biologie des tissus conjonctifs, C.H.U. du Sart Tilman, Université de Liège.
Bull Mem Acad R Med Belg. 1996;151(3-4):243-9; discussion 250-2.
Human fibroblasts display a Ca2+ wave after irradiation with an electromagnetic field (EMF) of low intensity (100 to 900 microT) as seen by LASER confocal microscopy and excitation of Fluo 3. The number of excited cells is proportional to the intensity of EMF between 100 and 900 microT. Cellular activation by a dialysable serum factor is required to induce the Ca2+ wave. It also depends on extracellular Ca2+ and active tyrosine kinases and phospholipase C gamma.
通过激光共聚焦显微镜和Fluo 3荧光激发观察发现,人成纤维细胞在受到低强度(100至900微特斯拉)电磁场(EMF)照射后会出现钙离子波。在100至900微特斯拉之间,被激发的细胞数量与电磁场强度成正比。诱导钙离子波需要一种可透析的血清因子激活细胞。这也依赖于细胞外钙离子以及活性酪氨酸激酶和磷脂酶Cγ。