Picard P, Bourgoin-Grenèche M, Zivy M
Groupe d'Etude et de Contrôle des Variétés et des Semences, Guyancourt, France.
Electrophoresis. 1997 Jan;18(1):174-81. doi: 10.1002/elps.1150180131.
Four closely related durum wheat varieties were compared by computer-assisted analysis of two-dimensional electrophoretic maps of leaf proteins. A low inter-varietal polymorphism was revealed and seven reliable qualitatively varying proteins allowed rapid visual identification of genotypes. For numerous spots, presence/absence or quantitative variations were greatly affected by a batch effect. Several criteria that should be used to discard unreliable spots or gels a priori were reviewed. Nevertheless, it was shown that, provided that the experimental design allows the integration of the batch effect, screening for discriminant markers as well as computing distances based on protein quantity variations are possible and allow variety identification. Euclidean and Mahalanobis distances allowed variety discrimination and single gel classification with a minimum risk of error, not only by taking into account the quantitative variations in discriminant proteins selected by analysis of variance, but also by taking into account all reproducible spots. The possible applications of two-dimensional electrophoresis in variety identification are discussed.
通过对叶蛋白二维电泳图谱进行计算机辅助分析，比较了四个亲缘关系密切的硬粒小麦品种。结果显示品种间多态性较低，有七种可靠的定性变化蛋白可用于快速直观地鉴定基因型。对于众多斑点，其存在/缺失或定量变化受批次效应的影响很大。本文回顾了一些应 priori 用于舍弃不可靠斑点或凝胶的标准。然而，研究表明，只要实验设计能够整合批次效应，就可以筛选判别标记并基于蛋白质数量变化计算距离，从而实现品种鉴定。欧几里得距离和马氏距离不仅通过考虑方差分析所选判别蛋白的定量变化，还通过考虑所有可重复的斑点，实现了品种判别和单凝胶分类，且错误风险最小。本文还讨论了二维电泳在品种鉴定中的可能应用。
