Dalgaard P, Manfio G P, Goodfellow M
Danish Institute for Fisheries Research, Department of Seafood Research, Technical University, Lyngby, Denmark.
Zentralbl Bakteriol. 1997 Jan;285(2):157-68. doi: 10.1016/s0934-8840(97)80024-2.
Forty strains of luminous and non-luminous Photobacterium phosphoreum isolates from cod (Gadus morhua) and seven reference strains of psychrotolerant and mesophilic photobacteria were examined for 156 unit characters in a numerical taxonomic study. The fish strains were isolated from the intestines, from spoiled products and by using a specific detection method. The data were analysed using the similarity coefficient and the unweighted pair-group with arithmetic averages algorithm. In addition twenty-six of the fish isolates and five reference strains were analysed by Curie-point pyrolysis mass spectrometry. The mesophilic and psychrotolerant photobacteria were clearly separated in each of the analyses. Both procedures indicated that the spoilage isolates of P. phosphoreum had originated from the live fish as strains from the fish intestines and from the spoiled products were recovered in the same sub-groups. One sub-group of psychrotolerant P. phosphoreum strains, which was selected in modified atmosphere packed fish stored at low temperature, was also highlighted using each of the methods. The importance of classifying food spoilage bacteria has been shown and a simple key generated for the identification of luminous and non-luminous isolates of Photobacterium phosphoreum.
在一项数值分类学研究中，对从鳕鱼（鳕属）中分离出的40株发光和不发光的磷光弧菌菌株以及7株耐冷和嗜温发光细菌的参考菌株进行了156个单位性状的检测。这些鱼类菌株是从肠道、变质产品中分离得到的，并采用了特定的检测方法。使用相似系数和算术平均法的非加权配对组法对数据进行分析。此外，对26株鱼类分离株和5株参考菌株进行了居里点热解质谱分析。在每项分析中，嗜温和耐冷发光细菌都被明显区分开来。两种方法均表明，磷光弧菌的变质分离株起源于活鱼，因为从鱼肠道和变质产品中分离出的菌株属于同一亚组。在低温储存的气调包装鱼中选择的一个耐冷磷光弧菌菌株亚组，也通过每种方法得到了突出显示。已经证明了对食品腐败细菌进行分类的重要性，并生成了一个简单的检索表，用于鉴定磷光弧菌的发光和不发光分离株。
