Doco T, Williams P, Vidal S, Pellerin P
Institut National de la Recherche Agronomique, Unité de Recherches des Polymères et des Techniques Physico-Chimiques, Montpellier, France.
Carbohydr Res. 1997 Jan 2;297(2):181-6. doi: 10.1016/s0008-6215(96)00260-1.
Rhamnogalacturonan II (RG-II), a small complex pectic polysaccharide, is released from apple (Malus domestica), carrot (Daucus carota), and tomato (Solanum lycopersicum) by treatment with two commercial liquefying enzyme preparations. RG-II was isolated by size-exclusion chromatography from apple, tomato, and carrot juices obtained by enzymic liquefaction. All the RG-IIs contained the diagnostic sugars, apiose, 2-O-methyl-L-fucose, 2-O-methyl-D-xylose, aceric acid, Kdo and Dha. Glycosyl-linkage compositions of the neutral and acidic sugars, including aceric acid, were consistent with the hypothetical model described for sycamore RG-II confirming the conservation of RG-II in plants. Thus, when pectinolytic enzyme preparations are used to process fruits and vegetables, RG-II is released as a main soluble polysaccharide fraction while other pectic polysaccharides are heavily degraded.
鼠李半乳糖醛酸聚糖II（RG-II）是一种小分子复合果胶多糖，通过用两种商业液化酶制剂处理，可从苹果（苹果属）、胡萝卜（胡萝卜属）和番茄（番茄属）中释放出来。通过尺寸排阻色谱法从酶促液化得到的苹果汁、番茄汁和胡萝卜汁中分离出RG-II。所有的RG-II都含有标志性糖类，如芹糖、2-O-甲基-L-岩藻糖、2-O-甲基-D-木糖、乙酰基丙酮酸、Kdo和Dha。中性糖和酸性糖（包括乙酰基丙酮酸）的糖基连接组成与针对悬铃木RG-II描述的假设模型一致，证实了RG-II在植物中的保守性。因此，当使用果胶分解酶制剂加工水果和蔬菜时，RG-II作为主要的可溶性多糖组分被释放出来，而其他果胶多糖则被大量降解。
