Lüttge H, Heidelberg T, Stangier K, Thiem J, Bretting H
Zoologisches Institut und Zoologisches Museum, Hamburg, Germany.
Carbohydr Res. 1997 Jan 17;297(3):281-8. doi: 10.1016/s0008-6215(96)00277-7.
The specificity of an L-galactosyltransferase (L-Gal-T) from albumen glands of the snail Helix pomatia has been studied. This enzyme transfers L-Gal from GDP-L-Gal to various disaccharides with beta-linked D-Gal in terminal non-reducing position, forming an alpha-(1-->2) linkage. The subterminal residue and the type of interglycosidie linkage proved to be of minor importance. However, the branched trisaccharide beta-D-Gal-(1-->3)-[beta-D-Gal-(1-->6)]-beta-D-Gal-(1-->O)Me is a very poor acceptor. The specificity of the L-Gal-T correlates well with the equimolar occurrence of L-Gal and the structural element-->2)-Gal- (1-->found in the storage polysaccharide of this snail. Since L-Fuc is also transferred from its GDP-activated form, the membrane preparations of the albumen glands can be used to synthesize fucosylated oligosaccharides.
对苹果蜗牛（Helix pomatia）蛋白腺中的L-半乳糖基转移酶（L-Gal-T）的特异性进行了研究。该酶将GDP-L-半乳糖中的L-半乳糖转移到各种二糖上，这些二糖在末端非还原位置具有β-连接的D-半乳糖，形成α-(1→2)键。亚末端残基和糖苷键类型证明不太重要。然而，分支三糖β-D-半乳糖-(1→3)-[β-D-半乳糖-(1→6)]-β-D-半乳糖-(1→O)Me是一种非常差的受体。L-Gal-T的特异性与L-半乳糖的等摩尔出现以及在这种蜗牛的储存多糖中发现的结构元件→2)-半乳糖-(1→很好地相关。由于L-岩藻糖也从其GDP活化形式转移，蛋白腺的膜制剂可用于合成岩藻糖基化寡糖。
