Smith A V, Roeder G S
Department of Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
J Cell Biol. 1997 Mar 10;136(5):957-67. doi: 10.1083/jcb.136.5.957.
Mutants in the meiosis-specific RED1 gene of S. cerevisiae fail to make any synaptonemal complex (SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein, Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed axial elements present in a zip1 mutant, suggesting that Red1 is a component of the lateral elements of mature SCs. Anti-Red1 staining is confined to the cores of meiotic chromosomes and is not associated with the loops of chromatin that lie outside the SC. Analysis of the spo11 mutant demonstrates that Red1 localization does not depend upon meiotic recombination. The localization of Red1 has been compared with two other meiosis-specific components of chromosomes, Hop1 and Zip1; Zip1 serves as a marker for synapsed chromosomes. Double labeling of wild-type meiotic chromosomes with anti-Zip1 and anti-Red1 antibodies demonstrates that Red1 localizes to chromosomes both before and during pachytene. Double labeling with anti-Hop1 and anti-Red1 antibodies reveals that Hop1 protein localizes only in areas that also contain Red1, and studies of Hop1 localization in a red1 null mutant demonstrate that Hop1 localization depends on Red1 function. These observations are consistent with previous genetic studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene chromosomes or in synapsed chromosomal regions.
酿酒酵母减数分裂特异性RED1基因的突变体无法形成任何联会复合体（SC）或SC的任何明显前体。使用特异性识别Red1蛋白的抗体，Red1已定位在减数分裂粗线期染色体上。Red1也定位于zip1突变体中存在的未联会轴向元件，这表明Red1是成熟SC侧向元件的一个组成部分。抗Red1染色局限于减数分裂染色体的核心，与位于SC外的染色质环无关。对spo11突变体的分析表明，Red1的定位不依赖于减数分裂重组。已将Red1的定位与染色体的另外两个减数分裂特异性成分Hop1和Zip1进行了比较；Zip1用作联会染色体的标记。用抗Zip1和抗Red1抗体对野生型减数分裂染色体进行双重标记表明，Red1在粗线期之前和期间都定位于染色体上。用抗Hop1和抗Red1抗体进行双重标记显示，Hop1蛋白仅定位于也含有Red1的区域，并且对red1缺失突变体中Hop1定位的研究表明，Hop1的定位取决于Red1的功能。这些观察结果与先前的遗传研究一致，表明Red1和Hop1直接相互作用。在粗线期染色体或联会染色体区域几乎没有Hop1蛋白。
