在E1-E3缺失的腺病毒载体中，E2转录单元的条件性抑制与体外病毒DNA复制和晚期基因表达的显著降低相关。
Conditional repression of the E2 transcription unit in E1-E3-deleted adenovirus vectors is correlated with a strong reduction in viral DNA replication and late gene expression in vitro.
作者信息
Rittner K, Schultz H, Pavirani A, Mehtali M
机构信息
Gene Therapy Department, Transgène S.A., Strasbourg, France.
出版信息
J Virol. 1997 Apr;71(4):3307-11. doi: 10.1128/JVI.71.4.3307-3311.1997.
Abstract
An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and late gene expression was observed in noncomplementing A549 cells, and a reduction in viral growth was seen in the E1-expressing 293 cells. In contrast, there was no repression in the presence of the regulator tetracycline. We propose that regulation by TetR-KRAB is a valuable tool with which to study the effects of viral gene expression in vitro.
摘要
已构建出一种表达杂交蛋白TetR-KRAB的E1-E3缺失重组腺病毒载体。在这种名为AdTG9562的病毒中，E2转录受顺式插入的TetR-KRAB和tetO序列调控。在无四环素的情况下，在非互补性A549细胞中观察到E2A基因表达、病毒DNA复制和晚期基因表达显著降低，在表达E1的293细胞中病毒生长也有所减少。相比之下，在存在调节因子四环素的情况下则没有抑制作用。我们认为，TetR-KRAB介导的调控是一种用于在体外研究病毒基因表达影响的有价值工具。
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