从紫色非硫细菌红假单胞菌属物种中克隆dnaK和dnaJ同源基因。

Cloning of dnaK and dnaJ homologous genes from a purple non-sulfur bacterium Rhodopseudomonas species.

作者信息

Momma K, Inui M, Yamagata H, Yukawa H

机构信息

Research Institute of Innovative Technology for the Earth, Kyoto, Japan.

出版信息

Biochim Biophys Acta. 1997 Feb 28;1350(3):235-9. doi: 10.1016/s0167-4781(96)00222-9.

DOI:10.1016/s0167-4781(96)00222-9

PMID:9061015

Abstract

The dnaK and dnaJ genes were isolated as a cluster from a purple non-sulfur phototrophic bacterium, Rhodopseudomonas species No. 7 by a polymerase chain reaction (PCR) based method. The deduced products of dnaK (631 amino acids) and dnaJ (379 amino acids) were 67% and 56% identical to the respective Escherichia coli gene products. The functions of DnaK and DnaJ could be confirmed by complementation of the respective E. coli mutants.

摘要

通过基于聚合酶链反应（PCR）的方法，从紫色非硫光合细菌红假单胞菌7号菌株中分离出dnaK和dnaJ基因簇。dnaK（631个氨基酸）和dnaJ（379个氨基酸）的推导产物分别与大肠杆菌相应基因产物有67%和56%的同源性。DnaK和DnaJ的功能可通过互补相应的大肠杆菌突变体得到证实。