通过诱导43 kDa侵袭质粒抗原ipaC检测致病性志贺氏菌和肠侵袭性大肠杆菌
Detection of virulent Shigella and enteroinvasive Escherichia coli by induction of the 43 kDa invasion plasmid antigen, ipaC.
作者信息
Shamlal R, Rajarathnam S, Sankaran K, Ramachandran V, Subrahmanyam Y V, Nair G B, Macaden R, Roy R K
机构信息
Astra Research Centre India, Bangalore, India.
出版信息
FEMS Immunol Med Microbiol. 1997 Feb;17(2):73-8. doi: 10.1111/j.1574-695X.1997.tb00998.x.
The invasion plasmid antigen, ipaC (43 kDa) of Shigella spp. and enteroinvasive Escherichia coli (EIEC) could be induced in vitro by growing them in the presence of Congo red. An enzyme-linked immunosorbent assay (ELISA) using antibodies to the 43 kDa protein of Shigella has been developed for specific detection of virulent Shigella spp and EIEC. The test is independent of initial isolation of individual colonies. As few as 10(2) CFU/ml of virulent Shigella present in mixed cultures could be detected and concurrently their susceptibility to antibiotics could be analysed after an initial growth of 8-16 h in Congo red-containing medium. The test may prove useful in the diagnosis and treatment of bacillary dysentery caused either by Shigella or EIEC through their rapid identification and proper antimicrobial therapy.
志贺氏菌属和肠侵袭性大肠杆菌（EIEC）的侵袭质粒抗原ipaC（43 kDa）可通过在刚果红存在的情况下培养而在体外诱导产生。已开发出一种使用针对志贺氏菌43 kDa蛋白的抗体的酶联免疫吸附测定（ELISA），用于特异性检测有毒力的志贺氏菌属和EIEC。该测试不依赖于单个菌落的初始分离。在含刚果红的培养基中初步生长8 - 16小时后，能够检测出混合培养物中低至10² CFU/ml的有毒力志贺氏菌，同时还可分析它们对抗生素的敏感性。该测试可能通过快速鉴定和适当的抗菌治疗，在由志贺氏菌或EIEC引起的细菌性痢疾的诊断和治疗中证明是有用的。
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