Floderus E, Pál T, Karlsson K, Lindberg A A
Department of Clinical Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
Eur J Clin Microbiol Infect Dis. 1995 Feb;14(2):111-7. doi: 10.1007/BF02111868.
BALB/c mice were immunised with water extracts made from an Escherichia coli K-12 strain harbouring the shigella invasion plasmid, and hybridomas secreting antibodies specific to invasion plasmid-coded antigens were selected. On Western blots, antibodies produced by one of these clones (MAIC-1) recognised a protein of 43 kDa, which is the molecular mass of invasion plasmid coded antigen C (IpaC). When used in enzyme immunoassay against whole bacterial cells or against proteins secreted by actively growing bacteria, MAIC-1 clearly differentiated between invasive and non-invasive strains. Testing 123 enteroinvasive and 139 non-enteroinvasive strains the MAIC-1 based assay proved to be highly specific and sensitive in recognising enteroinvasive isolates. This test could be an inexpensive and rapid alternative to cumbersome virulence assays and a helpful technique in identifying Shigella or enteroinvasive Escherichia coli isolates.
用携带志贺氏菌侵袭质粒的大肠杆菌K-12菌株制备的水提取物免疫BALB/c小鼠，并筛选出分泌针对侵袭质粒编码抗原的特异性抗体的杂交瘤。在蛋白质免疫印迹法中，这些克隆之一（MAIC-1）产生的抗体识别出一种43 kDa的蛋白质，这是侵袭质粒编码抗原C（IpaC）的分子量。当用于针对全菌细胞或针对活跃生长细菌分泌的蛋白质的酶免疫测定时，MAIC-1能够清楚地区分侵袭性菌株和非侵袭性菌株。通过检测123株侵袭性菌株和139株非侵袭性菌株，基于MAIC-1的检测方法在识别侵袭性分离株方面被证明具有高度特异性和敏感性。该检测方法可能是一种替代繁琐毒力检测的廉价且快速的方法，也是鉴定志贺氏菌或侵袭性大肠杆菌分离株的有用技术。
