采用反相高效液相色谱-紫外检测法分析血浆中的紫苏酸。

Analysis of perillic acid in plasma by reversed-phase high-performance liquid chromatography with ultraviolet detection.

作者信息

Ikechukwu Ezennia E, Phillips L R, Wolfe T L, Esmail Tabibi S

机构信息

Drug Formulation Laboratory, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Jan 24;688(2):354-8. doi: 10.1016/s0378-4347(96)00322-2.

DOI:10.1016/s0378-4347(96)00322-2

PMID:9061476

Abstract

A simple and sensitive isocratic high-performance liquid chromatographic (HPLC) method with UV detection for the quantitation of perillic acid, a major circulating metabolite of perillyl alcohol and d-limonene, in plasma is described. Sample preparation involved protein precipitation and subsequent transfer and dilution with 10 mM NaHCO3. The mobile phase consisted of acetonitrile (36%) and 0.05 M ammonium acetate buffer pH 5.0 (64%). Separations were achieved on a C18 column and the effluent monitored for UV absorption at the analytes' respective UVmax. Separation was excellent with no interference from endogenous plasma constituents. This method was found suitable for quantifying drug concentrations in the range of 0.25 to 200.0 micrograms/ml using a 0.05-ml plasma sample, and was used to study the plasma pharmacokinetics of perillic acid in mice.

摘要

本文描述了一种简单灵敏的等度高效液相色谱（HPLC）方法，采用紫外检测法对血浆中紫苏酸进行定量分析。紫苏酸是紫苏醇和d-柠檬烯的主要循环代谢产物。样品制备包括蛋白质沉淀，随后用10 mM碳酸氢钠进行转移和稀释。流动相由乙腈（36%）和pH 5.0的0.05 M醋酸铵缓冲液（64%）组成。在C18柱上实现分离，并在分析物各自的紫外最大吸收波长处监测流出物的紫外吸收。分离效果极佳，无内源性血浆成分干扰。该方法适用于使用0.05 ml血浆样品定量0.25至200.0微克/毫升范围内的药物浓度，并用于研究小鼠体内紫苏酸的血浆药代动力学。