Wishart J M, Horowitz M, Need A G, Scopacasa F, Morris H A, Clifton P M, Nordin B E
Department of Medicine, Royal Adelaide Hospital, Australia.
Am J Clin Nutr. 1997 Mar;65(3):798-802. doi: 10.1093/ajcn/65.3.798.
The relations between calcium absorption, dietary calcium intake, 1,25-dihydroxyvitamin D3 (calcitriol), and vitamin D receptor (VDR) gene polymorphisms were evaluated in 99 healthy women who were approaching menopause (mean age: 47 y, range: 43-53 y). Dietary calcium was assessed by food-frequency questionnaire and calcium absorption was measured by a single-isotope radiocalcium test. VDR alleles were classified according to the presence (b, t, a) or absence (B, T, A) of the BsmI, TaqI, and ApaI restriction enzyme cutting sites. Radiocalcium absorption was positively related to serum calcitriol (r = 0.23, P < 0.05) and inversely related to dietary calcium intake (r = -0.26, P < 0.01). There was, however, no significant relation (r = 0.10) between serum calcitriol concentrations and dietary calcium. Radiocalcium absorption was higher in the bbaaTT haplotype (P < 0.05) and the aa genotype (P < 0.05), polymorphisms said to be associated with a higher bone density. We conclude that serum calcitriol and dietary calcium are independent determinants of calcium absorption in premenopausal women and that VDR gene polymorphisms influence calcium absorption.
在99名接近更年期的健康女性（平均年龄：47岁，范围：43 - 53岁）中，评估了钙吸收、膳食钙摄入量、1,25 - 二羟基维生素D3（骨化三醇）和维生素D受体（VDR）基因多态性之间的关系。通过食物频率问卷评估膳食钙，并通过单同位素放射性钙试验测量钙吸收。根据BsmI、TaqI和ApaI限制性内切酶切割位点的存在（b、t、a）或不存在（B、T、A）对VDR等位基因进行分类。放射性钙吸收与血清骨化三醇呈正相关（r = 0.23，P < 0.05），与膳食钙摄入量呈负相关（r = -0.26，P < 0.01）。然而，血清骨化三醇浓度与膳食钙之间没有显著关系（r = 0.10）。bbaaTT单倍型（P < 0.05）和aa基因型（P < 0.05）的放射性钙吸收较高，这些多态性据说与较高的骨密度有关。我们得出结论，血清骨化三醇和膳食钙是绝经前女性钙吸收的独立决定因素，并且VDR基因多态性影响钙吸收。
