Upadhyay S N, Dey T B, Ghose A
Radiation Biology Department, Institute of Nuclear Medicine & Allied Sciences, Delhi, India.
Indian J Exp Biol. 1996 May;34(5):403-7.
Entrapment of 5-hydroxyl-L-tryptophan (HT) in erythrocyte ghost prepared by hypotonic method and high voltage electric discharge method are nearly same. Release of HT with beta-aminoethylisothiuronium bromide hydrobromide (HT + AET) in in vitro system is rapid but only a portion of the entrapped amount is released. Release of HT + AET in serum marginally increases at 2 hr. Compared to release in in vitro medium the release in serum is less. Survival studies with Swiss albino mice indicates that compared to HT alone, the combination of HT + AET shows about 9 times percentage survival. The same combination in the encapsulated form show comparable percentage survival though the amount needed is 1/200th times compared to free form.
通过低渗法和高压放电法制备的红细胞影对5-羟基-L-色氨酸（HT）的包封率几乎相同。在体外系统中，HT与氢溴酸β-氨基乙基异硫脲（HT + AET）的释放速度很快，但仅释放了部分包封量。HT + AET在血清中的释放在2小时时略有增加。与在体外培养基中的释放相比，血清中的释放较少。对瑞士白化小鼠的存活研究表明，与单独使用HT相比，HT + AET组合的存活率约高9倍。包封形式的相同组合显示出相当的存活率，尽管所需量与游离形式相比为1/200。
