Li X, Rawn J, DeCamp M M, Mentzer S J
Laboratory of Immunophysiology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Hybridoma. 1996 Feb;15(1):43-7. doi: 10.1089/hyb.1996.15.43.
Cell adhesion is a complex biophysical process that plays a central role in immunophysiology. Because of the complex force-energy relationships involved, insights into the mechanism of cell adhesion largely depend on comparative measurements. In this report, we describe a comparative approach to the measurement of cell adhesion under conditions of flow. The assay system perfuses fluorescently labeled lymphocytes over a cell monolayer in commercially available multiwell culture plates. The fluorescently labeled cells demonstrate a reproducible flow pattern within the well. Videomicroscopic recordings of cell movement have demonstrated rolling behavior over a wide range of cell velocities. This technique permits the measurement of cell adhesion over a variety of flow velocities, time courses, and treatment conditions. The ability to vary treatment conditions and provide multiple parallel conditions suggests the utility of this approach in the development of monoclonal antibodies (MAb) recognizing cell adhesion molecules.
细胞黏附是一个复杂的生物物理过程，在免疫生理学中起着核心作用。由于涉及复杂的力-能量关系，对细胞黏附机制的深入了解很大程度上依赖于比较测量。在本报告中，我们描述了一种在流动条件下测量细胞黏附的比较方法。该检测系统在市售的多孔培养板中，将荧光标记的淋巴细胞灌注到细胞单层上。荧光标记的细胞在孔内呈现出可重复的流动模式。细胞运动的视频显微镜记录显示，在很宽的细胞速度范围内都有滚动行为。这项技术能够在各种流速、时间进程和处理条件下测量细胞黏附。改变处理条件并提供多个平行条件的能力表明，这种方法在开发识别细胞黏附分子的单克隆抗体（MAb）方面具有实用性。
