Madden K, Sheu Y J, Baetz K, Andrews B, Snyder M
Department of Biology, Yale University, Post Office Box 208103, New Haven, CT 06520-8103, USA.
Science. 1997 Mar 21;275(5307):1781-4. doi: 10.1126/science.275.5307.1781.
Protein kinase C (PKC) signaling is highly conserved among eukaryotes and has been implicated in the regulation of cellular processes such as cell proliferation and growth. In the budding yeast, PKC1 functions to activate the SLT2(MPK1) mitogen-activated protein (MAP) kinase cascade, which is required for the maintenance of cell integrity during asymmetric cell growth. Genetic studies, coimmunoprecipitation experiments, and analysis of protein phosphorylation in vivo and in vitro indicate that the SBF transcription factor (composed of Swi4p and Swi6p), an important regulator of gene expression at the G1 to S phase cell cycle transition, is a target of the Slt2p(Mpk1p) MAP kinase. These studies provide evidence for a direct role of the PKC1 pathway in the regulation of the yeast cell cycle and cell growth and indicate that conserved signaling pathways can act to control key regulators of cell division.
蛋白激酶C（PKC）信号传导在真核生物中高度保守，并参与细胞增殖和生长等细胞过程的调控。在芽殖酵母中，PKC1的功能是激活SLT2（MPK1）丝裂原活化蛋白（MAP）激酶级联反应，这在不对称细胞生长过程中维持细胞完整性是必需的。遗传学研究、免疫共沉淀实验以及体内和体外蛋白质磷酸化分析表明，SBF转录因子（由Swi4p和Swi6p组成）是G1到S期细胞周期转换时基因表达的重要调节因子，是Slt2p（Mpk1p）MAP激酶的一个靶点。这些研究为PKC1途径在酵母细胞周期和细胞生长调控中的直接作用提供了证据，并表明保守的信号传导途径可以作用于控制细胞分裂的关键调节因子。
