Moser T, Neher E
Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, D-37077, Göttingen, Germany.
J Neurosci. 1997 Apr 1;17(7):2314-23. doi: 10.1523/JNEUROSCI.17-07-02314.1997.
We report here that brief depolarizations such as action potentials trigger exocytosis in thin mouse adrenal slices. The secretory rates obtained in membrane capacitance recordings from chromaffin cells in slices are faster than those observed in isolated cells. Fast exocytosis in slices is attributable to the rapid release of a small pool of vesicles. The pool recovers from depletion with a time constant of 10 sec. Recruitment of the rapidly released vesicles is strongly hindered by the fast Ca2+ chelator BAPTA and much less by the slower chelator EGTA. We suggest that these vesicles are located in close proximity to Ca2+ channels. Spatial coupling of Ca2+ entry and exocytosis may be sensitive to cell isolation and culture.
我们在此报告，短暂的去极化（如动作电位）可触发薄的小鼠肾上腺切片中的胞吐作用。从切片中的嗜铬细胞进行膜电容记录所获得的分泌速率比在分离细胞中观察到的要快。切片中的快速胞吐作用归因于一小部分囊泡的快速释放。这部分囊泡池从耗尽状态恢复的时间常数为10秒。快速释放的囊泡的募集受到快速Ca2+螯合剂BAPTA的强烈阻碍，而受到较慢的螯合剂EGTA的阻碍则小得多。我们认为这些囊泡位于靠近Ca2+通道的位置。Ca2+内流与胞吐作用的空间偶联可能对细胞分离和培养敏感。
