Mashimo J, Maniwa R, Sugino H, Nose K
Department of Microbiology, Showa University School of Pharmaceutical Sciences, Shinagawa-ku, Tokyo, Japan.
Cancer Lett. 1997 Feb 26;113(1-2):213-9. doi: 10.1016/s0304-3835(97)04700-9.
TSC-36, a TGF beta1-inducible gene, encodes a polypeptide that shows significant similarity to SPARC (secreted protein rich in cysteine), and follistatin, an activin-binding protein. The expression of the TSC-36 gene was reported to be extinguished in v-ras-transformed mouse fibroblastic cells, and also was found also to be abrogated in cells transformed with v-myc. The level of expression was, however, not affected in cells transformed with v-src, v-abl, or v-raf. The TSC-36 cDNA was first isolated from mouse cells, and recently its rat and human homologues have been reported which show striking similarity with each other. In various human tumor cells, TSC-36 mRNA was almost undetectable. TSC-36 mRNA was detected in various mouse organs, but its level was the highest in the lung. TSC-36 mRNA level was the highest in the lung among mouse organs, and on in situ hybridization, the TSC-36 transcript was detected in the alveolar epithelium but not in the bronchial epithelium. These features suggest possible usage of TSC-36 as one of the markers in human tumors.
TSC - 36是一种转化生长因子β1诱导基因，编码一种与富含半胱氨酸的分泌蛋白（SPARC）以及激活素结合蛋白卵泡抑素具有显著相似性的多肽。据报道，TSC - 36基因的表达在v - ras转化的小鼠成纤维细胞中消失，并且在用v - myc转化的细胞中也被发现被消除。然而，在用v - src、v - abl或v - raf转化的细胞中，其表达水平不受影响。TSC - 36 cDNA最初从小鼠细胞中分离出来，最近已经报道了它的大鼠和人类同源物，它们彼此之间显示出惊人的相似性。在各种人类肿瘤细胞中，几乎检测不到TSC - 36 mRNA。在各种小鼠器官中检测到了TSC - 36 mRNA，但其水平在肺中最高。在小鼠器官中，肺中的TSC - 36 mRNA水平最高，并且在原位杂交中，在肺泡上皮中检测到了TSC - 36转录本，但在支气管上皮中未检测到。这些特征表明TSC - 36有可能作为人类肿瘤的标志物之一。
