Challacombe S J, Rahman D, O'Hagan D T
Department of Oral Medicine and Pathology, UMDS, Guy's Hospital, London, UK.
Vaccine. 1997 Feb;15(2):169-75. doi: 10.1016/s0264-410x(96)00159-4.
The aims of this study were to determine whether oral immunization with microparticles might lead to a common mucosal response including vaginal secretions. Female Balb/c mice were immunized orally with microparticles containing ovalbumin at 0 and 4 weeks or with soluble antigen. Antibody responses were assayed by ELISA in saliva, gut washings, vaginal washings and serum, and antibody producing cells were assayed by ELISPOT in salivary glands and nasal cavity. After primary immunization, IgA antibodies were detected in vaginal washings, saliva and in gut washings which were significantly greater than those detected with soluble antigen (P < 0.01). After secondary immunization, greatly enhanced antibody titres were found in three fluids. The specific activity (antibody per microgram IgA) of antibodies in vaginal fluid and saliva was significantly greater than in serum or gut wash (P < 0.01). Oral immunization also resulted in the development of antibody forming cells in salivary glands and in nasal associated mucosal tissue. Immunization with microparticles containing antigen should prove useful in immunization against infections affecting a number of different mucosal surfaces.
本研究的目的是确定用微粒进行口服免疫是否会引发包括阴道分泌物在内的共同黏膜反应。雌性Balb/c小鼠在0周和4周时用含卵清蛋白的微粒进行口服免疫，或用可溶性抗原进行免疫。通过ELISA检测唾液、肠道灌洗液、阴道灌洗液和血清中的抗体反应，通过ELISPOT检测唾液腺和鼻腔中的抗体产生细胞。初次免疫后，在阴道灌洗液、唾液和肠道灌洗液中检测到IgA抗体，其含量显著高于用可溶性抗原检测到的抗体（P < 0.01）。二次免疫后，在三种液体中发现抗体滴度大大提高。阴道液和唾液中抗体的比活性（每微克IgA的抗体量）显著高于血清或肠道灌洗液（P < 0.01）。口服免疫还导致唾液腺和鼻相关黏膜组织中抗体形成细胞的发育。用含抗原的微粒进行免疫应证明对针对影响多个不同黏膜表面的感染进行免疫是有用的。
