Suzuki M, Kurono Y, Kodama S, Shigemi H, Mogi G
Department of Otolaryngology, Oita Medical University, Japan.
Acta Otolaryngol. 1998 Nov;118(6):864-9. doi: 10.1080/00016489850182594.
BALB/c mice were orally immunized with liposomes containing outer membrane proteins (OMPs) isolated from nontypeable Haemophilus influenzae (NTHi) and GM-53 as an adjuvant daily for 14 days. Anti-OMP IgA antibody titers in nasal wash, saliva, and fecal extract were significantly increased after the immunization. Although anti-OMP IgM and IgG antibodies were detected in serum, serum IgA antibodies specific to OMPs were not found. Enzyme-linked immunospot assay showed an increased number of OMP-specific IgA-secreting cells in nasal passages, intestinal lamina propria lymphocytes, and the spleen. Following oral immunization, a suspension of live NTHi was injected into the nose; nasal washes were collected 12 h after the inoculation. The number of NTHi in nasal washes was significantly reduced in mice immunized with liposomes containing OMPs and GM-53 compared to that in mice immunized with liposomes containing GM-53 alone. There was a significant negative correlation between the number of NTHi and anti-OMP IgA antibody titers in nasal washes. These findings suggest that antigen-specific IgA responses in the nose can be induced by oral immunization with OMPs and might be associated with the ability to clear NTHi from the nose.
将含有从不可分型流感嗜血杆菌（NTHi）分离的外膜蛋白（OMPs）的脂质体与GM-53作为佐剂，每天对BALB/c小鼠进行口服免疫，持续14天。免疫后，鼻洗液、唾液和粪便提取物中的抗OMP IgA抗体滴度显著升高。虽然在血清中检测到了抗OMP IgM和IgG抗体，但未发现OMP特异性血清IgA抗体。酶联免疫斑点试验显示，鼻腔通道、肠道固有层淋巴细胞和脾脏中OMP特异性IgA分泌细胞数量增加。口服免疫后，将活的NTHi悬液注入鼻腔；接种后12小时收集鼻洗液。与仅用含GM-53的脂质体免疫的小鼠相比，用含OMPs和GM-53的脂质体免疫的小鼠鼻洗液中NTHi的数量显著减少。鼻洗液中NTHi的数量与抗OMP IgA抗体滴度之间存在显著的负相关。这些发现表明，口服OMP免疫可诱导鼻腔中的抗原特异性IgA反应，并且可能与从鼻腔清除NTHi的能力有关。
