Nawata S, Abolhoda A, Ross H M, Brooks A, Burt M E
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Ann Thorac Surg. 1997 Mar;63(3):796-9. doi: 10.1016/s0003-4975(96)01372-0.
A model of isolated single-lung perfusion in the rat has been established in our laboratory to study the chemotherapeutic treatment of pulmonary metastases. A sequential bilateral isolated lung perfusion model was designed to investigate the feasibility of staged perfusions in the rat.
Twenty-four Fischer rats were randomized into three experimental groups of 8 rats each. All rats underwent left isolated lung perfusion. One, 2, or 3 weeks later, the rats in groups I, II, and III, respectively, underwent contralateral (right) perfusion. Five control animals (group IV) underwent sequential bilateral sham thoracotomies 1 week apart. Arterial blood gas analysis was performed 1 week after the second operation in the rats in groups I and IV.
All animals survived the first operation, with 100% (8/8), 75% (6/8), and 100% (8/8) of the animals in perfusion groups I, II, and III, respectively, surviving the second operation. All control animals (group IV) survived the second sham thoracotomy. Arterial blood gas analysis did not show a significant difference in the oxygen or carbon dioxide partial pressure or the pH between group I and IV (p = 0.32, 0.96, and 0.76, respectively).
Our experiments demonstrate that sequential bilateral isolated lung perfusion is safe in and well tolerated by the rat. This model can be used to investigate the safety and efficacy of staged perfusions with chemotherapeutic agents in the treatment of bilateral pulmonary metastases in the rat.
我们实验室已建立大鼠单肺隔离灌注模型，用于研究肺转移瘤的化疗。设计了一种序贯双侧肺隔离灌注模型，以研究大鼠分期灌注的可行性。
24只Fischer大鼠随机分为3个实验组，每组8只。所有大鼠均接受左肺隔离灌注。1周、2周或3周后，分别对第I、II和III组大鼠进行对侧（右肺）灌注。5只对照动物（第IV组）相隔1周进行序贯双侧假开胸手术。对第I组和第IV组大鼠在第二次手术后1周进行动脉血气分析。
所有动物均在第一次手术中存活，第I、II和III灌注组分别有100%（8/8）、75%（6/8）和100%（8/8）的动物在第二次手术中存活。所有对照动物（第IV组）均在第二次假开胸手术中存活。动脉血气分析显示，第I组和第IV组之间的氧分压、二氧化碳分压或pH值无显著差异（分别为p = 0.32、0.96和0.76）。
我们的实验表明，序贯双侧肺隔离灌注在大鼠中是安全的，且耐受性良好。该模型可用于研究在大鼠双侧肺转移瘤治疗中使用化疗药物进行分期灌注的安全性和有效性。
