Kubota T, Kase S, Otani Y, Watanabe M, Teramoto T, Kitajima M
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Anticancer Res. 1997 Jan-Feb;17(1B):725-8.
In order to investigate the combined antitumor activity of 5-fluorouracil (5-FU), and recombinant human interferon alpha 2a (IFN alpha) or human fibroblastoid interferon beta (IFN beta), the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay was carried out using a cultured human colon cancer cell line (C-1) and fresh surgical specimens of gastric and colon carcinomas. IFNs did not show positive antitumor activity against C-1 cells, whereas 5-FU showed time- and concentration-dependent antitumor activity against C-1 cells. Furthermore, the antitumor activity of 5-FU on C-1 cells was augmented by IFN alpha or beta. When 5-FU (50 micrograms/ml) with IFN alpha (50 IU/ml). or IFN beta (50 IU/ml) was applied for the MTT assay with 48 hours incubation of fresh surgical specimens of gastric and colon carcinomas, the inhibition rates increased by 10% in 9 of 21 gastric specimens and in 18 of 36 colon carcinomas for IFN alpha (47.4% or 27/57), and in 8 of 15 gastric specimens and in 15 of 28 colon carcinomas for IFN beta (53.5% or 23/43). These results suggest that the chemosensitivity to 5-FU of human gastric and colon carcinomas is increased in the presence of IFNs, without involvement of the host-mediated immune system, and that this combined effect can be predicted by the MTT assay in vitro.
为了研究5-氟尿嘧啶（5-FU）与重组人α2a干扰素（IFNα）或人成纤维细胞样β干扰素（IFNβ）的联合抗肿瘤活性，采用培养的人结肠癌细胞系（C-1）以及胃癌和结肠癌的新鲜手术标本进行了3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H四氮唑溴盐（MTT）试验。IFN对C-1细胞未显示出阳性抗肿瘤活性，而5-FU对C-1细胞显示出时间和浓度依赖性的抗肿瘤活性。此外，IFNα或IFNβ增强了5-FU对C-1细胞的抗肿瘤活性。当将含5-FU（50微克/毫升）与IFNα（50国际单位/毫升）或IFNβ（50国际单位/毫升）用于胃癌和结肠癌新鲜手术标本的MTT试验，并孵育48小时时，对于IFNα，在21例胃癌标本中有9例、36例结肠癌标本中有18例抑制率提高了10%（47.4%或27/57）；对于IFNβ，在15例胃癌标本中有8例、28例结肠癌标本中有15例抑制率提高了10%（53.5%或23/43）。这些结果表明，在不存在宿主介导的免疫系统参与的情况下，IFN可增强人胃癌和结肠癌对5-FU的化学敏感性，并且这种联合效应可通过体外MTT试验预测。
