Wollenberg B, Jan N V, Pitzke P, Reiter W, Stieber P
Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig-Maximilians-University of Munich, Germany.
Anticancer Res. 1997 Jan-Feb;17(1A):413-8.
Inactivation of the p53 gene occurs in various tumors favouring the accumulation of genetic aberration. It is assumed that p53 protein can become immunogenic in stimulating the production of circulating anti p53 antibodies. Sera from 97 patients with primary and 30 patients with recurrent squamous cell carcinoma of the head and neck were examined for p53 autoantibodies with an enzyme-linked immunosorbent assay (ELISA). Sera from 42 patients with benign ENT-diseases and 28 healthy smokers served as the control. 38.1% (37/97) of the patients with primary and 36.6% (11/30) with recurrent SCCHN had autoantibodies to p53 in their serum. The evidence of p53 antibodies was not dependent on histological grading, T- or UICC stage of the disease. 24.2% (17/70) of the control group also had autoantibodies to p53 in the serum. The values of the control group and the patient group are so closely related, that p53 autoantibodies can not serve as a marker of malignancy. The indication for p53 measurement by ELISA should be very closely defined and limited.
p53基因失活出现在各种肿瘤中，这有利于遗传畸变的积累。据推测，p53蛋白在刺激循环抗p53抗体产生时可具有免疫原性。采用酶联免疫吸附测定（ELISA）法检测了97例原发性头颈鳞状细胞癌患者和30例复发性头颈鳞状细胞癌患者血清中的p53自身抗体。42例耳鼻喉科良性疾病患者和28例健康吸烟者的血清作为对照。原发性患者中有38.1%（37/97）、复发性头颈部鳞状细胞癌患者中有36.6%（11/30）血清中存在p53自身抗体。p53抗体的存在不依赖于疾病的组织学分级、T分期或国际抗癌联盟（UICC）分期。对照组中也有24.2%（17/70）的人血清中存在p53自身抗体。对照组和患者组的值密切相关，以至于p53自身抗体不能作为恶性肿瘤的标志物。通过ELISA法检测p53的指征应非常严格地界定和限制。
