Maass J D, Gottschlich S, Goeroegh T, Bruhn T, Lippert B M, Paulsen J I, Werner J A
Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany.
Anticancer Res. 1997 Jan-Feb;17(1A):473-8.
p53 mutations are amongst the most prevalent alterations in human cancer. Overexpression of p53 is usually caused by mutation and is observed in a high percentage of squamous cell carcinomas of the head and neck (SCCHN). Fifty two fresh samples of SCCHN were examined for p53 overexpression and 23 tumor-free tonsils served as controls. Using the monoclonal antibody Pab 1801 a refined ELISA technique was employed. In contrast to established ELISA procedures tumor tissue was pulverized at -80 degrees C prior to the actual ELISA (ELISA I). Comparative p53 detection was carried out by immunohistochemistry (IHC) and a commercially available ELISA kit (ELISA II). The modified ELISA I revealed p53 overexpression in 48 tumor samples (92%). p53 detection was obtained in 26 cases (50%) with ELISA II and with IHC 39 stained positive for p53 (75%). All of the controls were negative for p53 with ELISA and IHC. The p53 staining in IHC showed a significant correlation with the grading of the tumor. The ELISA results of the p53 overexpression did not show any correlation with tumor size or stage and rate of metastasis or other clinical parameters. This ELISA represents a more sensitive detection method of p53 than any other technique so far. It improves on former ELISA and IHC results on p53 overexpression in squamous cell carcinoma of the head and neck and underlines the involvement and the importance of the p53 tumor suppressor protein in carcinogenesis of head and neck cancer.
p53突变是人类癌症中最常见的改变之一。p53的过表达通常由突变引起，并且在高比例的头颈部鳞状细胞癌（SCCHN）中观察到。对52份SCCHN新鲜样本进行p53过表达检测，23份无肿瘤的扁桃体作为对照。使用单克隆抗体Pab 1801采用了一种改良的ELISA技术。与既定的ELISA程序不同，在实际ELISA（ELISA I）之前，肿瘤组织在-80℃下研磨。通过免疫组织化学（IHC）和市售ELISA试剂盒（ELISA II）进行p53的比较检测。改良的ELISA I在48个肿瘤样本（92%）中检测到p53过表达。ELISA II在26例（50%）中检测到p53，IHC检测到39例p53染色阳性（75%）。所有对照在ELISA和IHC检测中p53均为阴性。IHC中的p53染色与肿瘤分级显著相关。p53过表达的ELISA结果与肿瘤大小、分期、转移率或其他临床参数均无相关性。这种ELISA是迄今为止比任何其他技术都更灵敏的p53检测方法。它改进了先前关于头颈部鳞状细胞癌中p53过表达的ELISA和IHC结果，并强调了p53肿瘤抑制蛋白在头颈部癌发生过程中的参与和重要性。
