McCormick G T, Sanchez R M
Int J Lepr Other Mycobact Dis. 1979 Sep;47(3):495-9.
Various mycobacteria were tested for their ability to retain acid-fastness after treatment with pyridine: a) Mycobacterium leprae separated from organs of 20 experimentally infected armadillos (which were sacrificed); b) M. leprae separated from a biopsy of a lepromatous patient; c) direct smears of lepromatous tissues from armadillos; d) eighteen cultivable mycobacteria obtained from the American Type Culture Collection (ATCC); E) cultivatable mycobacteria separated from the lymph nodes of a wild-caught armadillo and also the same organism grown in culture and Skinsnes' alleged M. leprae culture. A loss of acid-fastness was observed microscopically from M. leprae separated from experimentally infected armadillo tissues, M. leprae separated from a lepromatous patient biopsy, and M. leprae found in direct smears prepared from infected armadillo tissues. The eighteen cultivatable mycobacteria from ATCC, cultivatable mycobacteria separated from the tissue of a wild-caught armadillo (and also grown in culture) and Skinsnes' alleged M. leprae culture retained their acid-fastness. Testing of pyridine extractability of acid-fastness combined with those of D-DOPA oxidase testing proved to be extremely reliable in our laboratory in differentiating M. leprae from other mycobacteria.
对各种分枝杆菌进行了检测，以观察它们在用吡啶处理后保持抗酸性的能力：a）从20只经实验感染的犰狳（已处死后）的器官中分离出的麻风分枝杆菌；b）从一名瘤型麻风患者的活检组织中分离出的麻风分枝杆菌；c）犰狳瘤型麻风组织的直接涂片；d）从美国典型培养物保藏中心（ATCC）获得的18种可培养分枝杆菌；e）从一只野生捕获的犰狳的淋巴结中分离出的可培养分枝杆菌，以及在培养物中生长的同一菌株和斯金斯内斯所谓的麻风分枝杆菌培养物。在显微镜下观察到，从经实验感染的犰狳组织中分离出的麻风分枝杆菌、从瘤型麻风患者活检组织中分离出的麻风分枝杆菌以及从感染犰狳组织制备的直接涂片中发现的麻风分枝杆菌失去了抗酸性。来自ATCC的18种可培养分枝杆菌、从野生捕获的犰狳组织中分离出的（并在培养物中生长的）可培养分枝杆菌以及斯金斯内斯所谓的麻风分枝杆菌培养物保持了它们的抗酸性。在我们实验室中，抗酸性的吡啶可提取性测试与D - DOPA氧化酶测试相结合，在区分麻风分枝杆菌与其他分枝杆菌方面被证明是极其可靠的。
