烟草叶片叶肉过氧化物酶。分离、纯化及某些特性
Tobacco leaf mesophyll peroxidase. Isolation, purification and some properties.
作者信息
Yung K H, Wong Y S, Choy Y M
出版信息
Int J Pept Protein Res. 1979 Jul;14(1):5-11. doi: 10.1111/j.1399-3011.1979.tb01914.x.
Abstract
Two isoperoxidases, P1 and P2, were isolated from tobacco mesophyll leaves by the combined methods of ammonium sulphate precipitation, PAGE and DEAE-cellulose column chromatography. The pH otpima for P1 and P2 were 7.0 and 7.5 respectively. The values of Km for H2O2 were 1.82 and 0.87 mM for P1 and P2. Results from this investigationsuggest that these isoperoxidases differ in their electrophoretic, catalytic and physical properties.
摘要
通过硫酸铵沉淀、聚丙烯酰胺凝胶电泳（PAGE）和二乙氨基乙基纤维素（DEAE -纤维素）柱色谱法相结合的方法，从烟草叶肉叶片中分离出两种过氧化物酶，P1和P2。P1和P2的最适pH分别为7.0和7.5。P1和P2对过氧化氢（H₂O₂）的米氏常数（Km）值分别为1.82和0.87 mM。该研究结果表明，这些过氧化物酶在电泳、催化和物理性质方面存在差异。
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