用于制备大型基因靶向载体的定点诱变方案。 - Suppr

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Protocol for site-directed mutagenesis in the preparation of large gene-targeting vectors.

作者信息

De Togni P

机构信息

University of Arkansas for Medical Sciences, Little Rock, USA.

出版信息

Biotechniques. 1997 Mar;22(3):426-8, 430. doi: 10.2144/97223bm10.

DOI:10.2144/97223bm10

PMID:9067013

Abstract

摘要

相似文献

Protocol for site-directed mutagenesis in the preparation of large gene-targeting vectors.

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Site-directed mutagenesis using uracil-containing double-stranded DNA templates and DpnI digestion.

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Construction of a vector for coupled in vitro transcription/translation.

Biotechniques. 1999 Sep;27(3):428-30, 434. doi: 10.2144/99273bm06.

Method of site-directed mutagenesis using long primer-unique site elimination and exonuclease III.

Biotechniques. 1997 Mar;22(3):430-4. doi: 10.2144/97223bm11.

Scanning mutagenesis to localize DNA-protein interactions.

Biotechniques. 1997 Mar;22(3):434-6. doi: 10.2144/97223bm12.

PCR-generated cross-over linkers for site-directed mutagenesis.

Biotechniques. 1997 Nov;23(5):827-30. doi: 10.2144/97235bm13.

Directional PCR cloning of multiple repeat sequences.

Biotechniques. 1996 Aug;21(2):198, 200. doi: 10.2144/96212bm05.

Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA.

Anal Biochem. 2003 Aug 15;319(2):335-6. doi: 10.1016/s0003-2697(03)00286-0.

Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis.

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Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis.

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Protocol for site-directed mutagenesis in the preparation of large gene-targeting vectors.

作者信息

De Togni P

机构信息

University of Arkansas for Medical Sciences, Little Rock, USA.

出版信息

Biotechniques. 1997 Mar;22(3):426-8, 430. doi: 10.2144/97223bm10.

DOI:10.2144/97223bm10

PMID:9067013

Abstract

摘要

相似文献

Protocol for site-directed mutagenesis in the preparation of large gene-targeting vectors.

Biotechniques. 1997 Mar;22(3):426-8, 430. doi: 10.2144/97223bm10.

Site-directed mutagenesis using uracil-containing double-stranded DNA templates and DpnI digestion.

Biotechniques. 1999 Oct;27(4):734-8. doi: 10.2144/99274st03.

Construction of a vector for coupled in vitro transcription/translation.

Biotechniques. 1999 Sep;27(3):428-30, 434. doi: 10.2144/99273bm06.

Method of site-directed mutagenesis using long primer-unique site elimination and exonuclease III.

Biotechniques. 1997 Mar;22(3):430-4. doi: 10.2144/97223bm11.

Scanning mutagenesis to localize DNA-protein interactions.

Biotechniques. 1997 Mar;22(3):434-6. doi: 10.2144/97223bm12.

PCR-generated cross-over linkers for site-directed mutagenesis.

Biotechniques. 1997 Nov;23(5):827-30. doi: 10.2144/97235bm13.

Directional PCR cloning of multiple repeat sequences.

Biotechniques. 1996 Aug;21(2):198, 200. doi: 10.2144/96212bm05.

Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA.

Anal Biochem. 2003 Aug 15;319(2):335-6. doi: 10.1016/s0003-2697(03)00286-0.

Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis.

Biotechniques. 1993 Nov;15(5):831-4.

Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis.

Methods Mol Biol. 2012;944:163-74. doi: 10.1007/978-1-62703-122-6_11.