使用改良的快速定点突变技术构建质粒载体的快速简便方法。
Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis.
作者信息
Bok Jin Woo, Keller Nancy P
机构信息
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
出版信息
Methods Mol Biol. 2012;944:163-74. doi: 10.1007/978-1-62703-122-6_11.
Abstract
Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a modified Quick-change site-directed mutagenesis method that allows for rapid and accurate construction of fungal transformation vectors.
摘要
质粒载体构建是分子微生物学的一个基本步骤，但往往是一个耗时的过程。对真菌基因组进行操作以表达基因来激活次级代谢产物的产生，通常需要以重复的方式构建质粒。在此，我们介绍一种改良的快速定点诱变方法，该方法能够快速、准确地构建真菌转化载体。
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