血小板生成素在体外与其他造血生长因子及生理性血小板激动剂协同作用以激活血小板。

Thrombopoietin is synergistic with other hematopoietic growth factors and physiologic platelet agonists for platelet activation in vitro.

作者信息

Wun T, Paglieroni T, Hammond W P, Kaushansky K, Foster D C

机构信息

Department of Internal Medicine, University of California at Davis School of Medicine, California, USA.

出版信息

Am J Hematol. 1997 Mar;54(3):225-32. doi: 10.1002/(sici)1096-8652(199703)54:3<225::aid-ajh9>3.0.co;2-y.

DOI:10.1002/(sici)1096-8652(199703)54:3<225::aid-ajh9>3.0.co;2-y

PMID:9067502

Abstract

Thrombopoietin (TPO) is the primary physiologic regulator of platelet production. The effect of TPO on platelet function, both alone and in combination with other hematopoietic growth factors, adenosine diphosphate (ADP), and epinephrine, was investigated using fluorescent-labeled antibodies to the activation-dependent antigen CD62 (P-selectin) and flow cytometry. TPO stimulated CD62 expression on normal human platelets, and this expression was completely inhibited by the soluble extracellular domain of the TPO receptor, MPL. The growth factors granulocyte colony-stimulating factor (G-CSF) and erythropoietin (EPO), but not interleukin-3 (IL-3) or stem-cell factor (SCF), also stimulated platelet activation. The combination of EPO, SCF, ADP, and epinephrine with TPO were synergistic for platelet CD62 expression. These data further support a role for TPO in modulating platelet function.

摘要

血小板生成素（TPO）是血小板生成的主要生理调节因子。使用针对活化依赖性抗原CD62（P-选择素）的荧光标记抗体和流式细胞术，研究了TPO单独以及与其他造血生长因子、二磷酸腺苷（ADP）和肾上腺素联合使用时对血小板功能的影响。TPO刺激正常人血小板上CD62的表达，并且该表达被TPO受体MPL的可溶性细胞外结构域完全抑制。生长因子粒细胞集落刺激因子（G-CSF）和促红细胞生成素（EPO）也刺激血小板活化，但白细胞介素-3（IL-3）或干细胞因子（SCF）则不然。EPO、SCF、ADP和肾上腺素与TPO联合使用对血小板CD62表达具有协同作用。这些数据进一步支持了TPO在调节血小板功能中的作用。