Tilgner J, Golembowski S, Kersten B, Sterry W, Jahn S
Department of Dermatology, Medical School (Charité), Humboldt-University Berlin, Germany.
Clin Exp Immunol. 1997 Mar;107(3):528-35. doi: 10.1046/j.1365-2249.1997.d01-960.x.
In this study we analysed the VH genes expressed in IgE-producing peripheral B cells in two patients with severe atopic dermatitis. We found a diverse antibody repertoire containing V(H) genes of five different VH families, no VH2 and 7 genes, with no biased usage of one particular VH gene family. Some VH germ-line segments were found to be used several times within the epsilon transcripts (V3-23, V3-30). Comparison of the IgE-encoding VH genes with the respective germ-line elements revealed the expression of nearly germ-line identical as well as somatically mutated VH genes. In these latter VH genes base substitutions were distributed over both complementarity determining and framework regions. Because of the similarities between the IgE committed B cell repertoire in atopic patients and the peripheral B cell repertoire in non-atopic individuals we conclude that the atopic VH gene repertoire probably reflects the result of an increased class switch of polyclonal B cells, including virgin and mature B cells, in response to a Th2 cytokine profile.
在本研究中，我们分析了两名重度特应性皮炎患者产生IgE的外周血B细胞中表达的VH基因。我们发现了一个多样化的抗体库，包含五个不同VH家族的V(H)基因，没有VH2和7基因，且没有对某一特定VH基因家族的偏向性使用。在ε转录本中发现一些VH种系片段被多次使用（V3-23、V3-30）。将编码IgE的VH基因与各自的种系元件进行比较，发现几乎与种系相同以及体细胞突变的VH基因均有表达。在这些后者的VH基因中，碱基替换分布在互补决定区和框架区。由于特应性患者中产生IgE的B细胞库与非特应性个体的外周血B细胞库之间存在相似性，我们得出结论，特应性VH基因库可能反映了多克隆B细胞（包括未成熟和成熟B细胞）响应Th2细胞因子谱而增加的类别转换的结果。
