Nelson T E, King J S, Bishop G A
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus 43210, USA.
J Comp Neurol. 1997 Mar 17;379(3):443-54. doi: 10.1002/(sici)1096-9861(19970317)379:3<443::aid-cne9>3.0.co;2-3.
The indirect antibody peroxidase-antiperoxidase technique was used to determine the laminar and lobular distribution of catecholaminergic afferents in the adult mouse, opossum, and cat cerebellum. A monoclonal antibody to tyrosine hydroxylase (TH) revealed a plexus of thin varicose fibers that exhibited a different density and distribution pattern for each species. In the cat, TH-immunoreactive fibers were sparsely distributed to all laminae, lobules, and nuclei of the cat cerebellum except for an area of elevated density in the ventral folia of lobules V and VI. In the opossum, TH-positive fibers were uniformly and densely distributed in the granule and Purkinje cell layers; they were more abundant in vermal lobules V-VI than in more anterior and posterior lobules, particularly I and X. Numerous TH-immunoreactive fibers were found in all four cerebellar nuclei of the opossum. In the mouse, TH-positive fibers formed a dense plexus within all cerebellar lobules, laminae, and nuclei. The mouse also exhibited numerous TH-immunoreactive Purkinje cells that were localized predominantly within vermal lobules VI-X, the paraflocculus, and flocculus. In addition to the interspecies differences in the distribution of catecholaminergic fibers within the cerebellum, comparison of this plexus to that previously described for serotonin in these species reveals that the relative densities and distribution patterns of catecholaminergic and serotoninergic fibers also vary between species. It is thus hypothesized that in each species a given monoamine has a unique net effect on cerebellar output that is determined by its effects on different neuronal populations within the cerebellum.
采用间接抗体过氧化物酶-抗过氧化物酶技术，确定成年小鼠、负鼠和猫小脑内儿茶酚胺能传入纤维的分层和小叶分布。抗酪氨酸羟化酶（TH）单克隆抗体显示出一束细的曲张纤维，每种动物的纤维密度和分布模式各不相同。在猫中，TH免疫反应性纤维稀疏地分布于猫小脑的所有层、小叶和核，但小叶V和VI腹侧叶中有一个密度升高的区域除外。在负鼠中，TH阳性纤维均匀且密集地分布在颗粒层和浦肯野细胞层；在蚓部小叶V-VI中比在前部和后部小叶，特别是I和X中更为丰富。在负鼠的所有四个小脑中都发现了大量TH免疫反应性纤维。在小鼠中，TH阳性纤维在所有小脑小叶、层和核内形成密集的丛。小鼠还表现出大量TH免疫反应性浦肯野细胞，主要位于蚓部小叶VI-X、旁绒球和绒球内。除了小脑内儿茶酚胺能纤维分布的种间差异外，将此丛与先前描述的这些动物血清素的丛进行比较发现，儿茶酚胺能纤维和血清素能纤维的相对密度和分布模式在不同物种之间也有所不同。因此推测，在每个物种中，一种特定的单胺对小脑输出具有独特的净效应，这是由其对小脑内不同神经元群体的作用决定的。
