噬菌体T4分子伴侣gp57A的分离与鉴定
Isolation and characterization of a molecular chaperone, gp57A, of bacteriophage T4.
作者信息
Matsui T, Griniuviené B, Goldberg E, Tsugita A, Tanaka N, Arisaka F
机构信息
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
J Bacteriol. 1997 Mar;179(6):1846-51. doi: 10.1128/jb.179.6.1846-1851.1997.
Abstract
A molecular chaperone of bacteriophage T4, gp57A, which facilitates the formation of the long and short tail fibers, was isolated and characterized by peptide analysis, sedimentation equilibrium, and circular dichroism (CD). Sequence analysis confirmed the predicted sequence of 79 amino acids from the nucleotide sequence of the gene with the N-terminal methionine removed. The result led to the conclusion that the apparent smaller molecular weight of 6,000 from Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the expected molecular weight of 8,710 was due to its abnormal electrophoretic behavior instead of cleavage or processing of the gene product. Estimation of the secondary structure from far-UV CD indicated a 94% alpha-helix content, which was in accord with the prediction from the primary structure. A sedimentation equilibrium study, on the other hand, revealed that gp57A assumes a tetrameric subunit structure.
摘要
噬菌体T4的一种分子伴侣gp57A，它促进长、短尾丝的形成，通过肽分析、沉降平衡和圆二色性（CD）进行分离和表征。序列分析证实了从去除N端甲硫氨酸的基因核苷酸序列预测的79个氨基酸序列。结果得出结论，在Tricine-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，表观分子量为6000，低于预期分子量8710，这是由于其异常的电泳行为，而非基因产物的切割或加工。远紫外CD对二级结构的估计表明，α-螺旋含量为94%，这与一级结构的预测一致。另一方面，沉降平衡研究表明，gp57A呈现四聚体亚基结构。
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本文引用的文献
1
The short tail-fiber of bacteriophage T4: molecular structure and a mechanism for its conformational transition.噬菌体T4的短尾丝：分子结构及其构象转变机制
Virology. 1993 May;194(1):117-27. doi: 10.1006/viro.1993.1241.
2
Molecular chaperone functions of heat-shock proteins.热休克蛋白的分子伴侣功能
Annu Rev Biochem. 1993;62:349-84. doi: 10.1146/annurev.bi.62.070193.002025.
3
Bacteriophage T4 encodes a co-chaperonin that can substitute for Escherichia coli GroES in protein folding.噬菌体T4编码一种共伴侣蛋白，它在蛋白质折叠过程中可替代大肠杆菌的GroES。
Nature. 1994 Apr 14;368(6472):654-6. doi: 10.1038/368654a0.
4
Estimation of globular protein secondary structure from circular dichroism.基于圆二色性的球状蛋白质二级结构估算
Biochemistry. 1981 Jan 6;20(1):33-7. doi: 10.1021/bi00504a006.
5
Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence.噬菌体T4基因57的核苷酸序列及推导的氨基酸序列。
Nucleic Acids Res. 1982 Feb 11;10(3):1105-12. doi: 10.1093/nar/10.3.1105.
6
Assembly of bacteriophage T4 tail fibers: identification and characterization of the nonstructural protein gp57.噬菌体T4尾丝的组装：非结构蛋白gp57的鉴定与表征
Mol Gen Genet. 1981;184(1):125-32. doi: 10.1007/BF00271208.
7
Form-determining function of the genes required for the assembly of the head of bacteriophage T4.噬菌体T4头部组装所需基因的形态决定功能。
J Mol Biol. 1970 Apr 14;49(1):99-113. doi: 10.1016/0022-2836(70)90379-7.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
A factor preventing the major head protein of bacteriophage T4 from random aggregation.一种阻止噬菌体T4主要头部蛋白随机聚集的因素。
J Mol Biol. 1970 Jan 14;47(1):69-85. doi: 10.1016/0022-2836(70)90402-x.
10
The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. I. Attachment and penetration.在电子显微镜下观察到的T2和T4噬菌体对大肠杆菌的感染。I. 吸附与侵入
Virology. 1967 Jun;32(2):279-97. doi: 10.1016/0042-6822(67)90277-2.
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