γ-氨基丁酸B型受体的表达克隆揭示了其与代谢型谷氨酸受体的相似性。

Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors.

作者信息

Kaupmann K, Huggel K, Heid J, Flor P J, Bischoff S, Mickel S J, McMaster G, Angst C, Bittiger H, Froestl W, Bettler B

机构信息

Novartis Pharma Inc., Research Department, Therapeutic Area Nervous System, Basel, Switzerland.

出版信息

Nature. 1997 Mar 20;386(6622):239-46. doi: 10.1038/386239a0.

DOI:10.1038/386239a0

PMID:9069281

Abstract

GABA (gamma-amino-butyric acid), the principal inhibitory neurotransmitter in the brain, signals through ionotropic (GABA(A)/ GABA(c)) and metabotropic (GABA(B)) receptor systems. Here we report the cloning of GABA(B) receptors. Photoaffinity labelling experiments suggest that the cloned receptors correspond to two highly conserved GABA(B) receptor forms present in the vertebrate nervous system. The cloned receptors negatively couple to adenylyl cyclase and show sequence similarity to the metabotropic receptors for the excitatory neurotransmitter L-glutamate.

摘要

γ-氨基丁酸（GABA）是大脑中的主要抑制性神经递质，通过离子型（GABA(A)/GABA(c)）和代谢型（GABA(B)）受体系统发挥信号传导作用。在此，我们报告GABA(B)受体的克隆。光亲和标记实验表明，克隆出的受体与脊椎动物神经系统中存在的两种高度保守的GABA(B)受体形式相对应。克隆出的受体与腺苷酸环化酶负向偶联，并且与兴奋性神经递质L-谷氨酸的代谢型受体在序列上具有相似性。