Sato A, Kitazawa H, Hayakawa H, Chida K, Iwata M
Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
J Antimicrob Chemother. 1997 Feb;39(2):217-22. doi: 10.1093/jac/39.2.217.
We studied the effect of AM-1155, a newly developed quinolone, against chronic airway infection with Pseudomonas aeruginosa using a previously described rat model. AM-1155 (25 mg/kg) or ciprofloxacin (25 mg/kg) or saline (controls) were injected s.c. for 14 days (from day 4 to day 17) after the inoculation of agar beads containing P. aeruginosa. The number of viable cells of intrapulmonary P. aeruginosa, histological findings of the lungs and immunoglobulin levels of serum and bronchoalveolar lavage fluid were examined in rats 11 and 18 days after the treatment. The findings indicated that the number of viable cells of P. aeruginosa in lungs was significantly decreased in the AM-1155- or ciprofloxacin-treated group compared with the non-treated control group. Histological examination in the non-treated control group showed hyperplasia of bronchus-associated lymphoid tissue as well as cellular infiltration in airways, but not prominently in the AM-1155- or ciprofloxacin-treated group. The IgG and IgA levels in serum and bronchoalveolar lavage fluid were significantly lower in the AM-1155- and ciprofloxacin-treated groups than in the control group. These in-vivo effects of AM-1155 were comparable to those of ciprofloxacin. These findings suggest that treatment with AM-1155 and ciprofloxacin suppressed excessive immune responses, preventing progression of airway damage in the chronic infectious state.
我们使用先前描述的大鼠模型，研究了新开发的喹诺酮类药物AM - 1155对铜绿假单胞菌慢性气道感染的影响。在接种含铜绿假单胞菌的琼脂珠后，皮下注射AM - 1155（25毫克/千克）或环丙沙星（25毫克/千克）或生理盐水（对照组），持续14天（从第4天至第17天）。在治疗后11天和18天检查大鼠肺内铜绿假单胞菌的活菌数量、肺组织学结果以及血清和支气管肺泡灌洗液中的免疫球蛋白水平。结果表明，与未治疗的对照组相比，AM - 1155或环丙沙星治疗组肺内铜绿假单胞菌的活菌数量显著减少。未治疗的对照组组织学检查显示支气管相关淋巴组织增生以及气道内细胞浸润，但在AM - 1155或环丙沙星治疗组中不明显。AM - 1155和环丙沙星治疗组血清和支气管肺泡灌洗液中的IgG和IgA水平显著低于对照组。AM - 1155的这些体内作用与环丙沙星相当。这些发现表明，AM - 1155和环丙沙星治疗可抑制过度的免疫反应，防止慢性感染状态下气道损伤的进展。
