Bergeron P, Douziech M, Daigle N, Blouin R
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
Biochem Biophys Res Commun. 1997 Feb 3;231(1):153-5. doi: 10.1006/bbrc.1997.6073.
ZPK is a recently described serine/threonine protein kinase that is thought to be involved in the regulation of cell proliferation and differentiation. To directly determine whether ZPK exhibits any effect on cell growth, NIH 3T3 fibroblasts were transfected with an expression vector harboring the murine ZPK cDNA. Stable expression of this construct led to a dramatic reduction in the proliferative capacity of these cells as measured by a colony formation assay in monolayer culture. By contrast, overexpression of a ZPK cDNA with a mutation in the ATP-binding domain did not affect clonal expansion of the transfected cells. These findings suggest that the ZPK gene may act as a negative regulator of cell growth and that this function may be mediated in part by the intrinsic kinase activity of the ZPK protein.
ZPK是一种最近被描述的丝氨酸/苏氨酸蛋白激酶，被认为参与细胞增殖和分化的调控。为了直接确定ZPK是否对细胞生长有任何影响，将携带小鼠ZPK cDNA的表达载体转染到NIH 3T3成纤维细胞中。通过单层培养中的集落形成试验测定，该构建体的稳定表达导致这些细胞的增殖能力显著降低。相比之下，ATP结合结构域发生突变的ZPK cDNA的过表达并不影响转染细胞的克隆扩增。这些发现表明，ZPK基因可能作为细胞生长的负调节因子，并且该功能可能部分由ZPK蛋白的内在激酶活性介导。
