大鼠脑中细胞色素P450 2E1的鉴定。
Identification of cytochrome P450 2E1 in rat brain.
作者信息
Yoo M, Ryu H M, Shin S W, Yun C H, Lee S C, Ji Y M, You K H
机构信息
Department of Biology, Keimyung University, Taegu, Korea.
出版信息
Biochem Biophys Res Commun. 1997 Feb 13;231(2):254-6. doi: 10.1006/bbrc.1997.6084.
We have isolated cDNA fragments that were originated from P450 2E1 in rat brain by PCR analysis. Their size matched up to what we expected based on the reported sequence of rat liver P450 2E1 mRNA. Internal structure of the longest first-round PCR products were investigated by Southern blot analysis and "nested" PCR. Their results confirmed that PCR products actually originated from P450 2E1 mRNA in rat brain. RT-PCR was also carried out using P450 2E1 specific primers and the size of the product was exactly as we expected for P450 2E1. These experimental evidences should clarify the presence of P450 2E1 in rat brain.
我们通过PCR分析从大鼠脑中分离出了源自P450 2E1的cDNA片段。它们的大小与基于报道的大鼠肝脏P450 2E1 mRNA序列所预期的相符。通过Southern印迹分析和“巢式”PCR研究了第一轮最长PCR产物的内部结构。它们的结果证实了PCR产物确实源自大鼠脑中的P450 2E1 mRNA。还使用P450 2E1特异性引物进行了RT-PCR，产物大小与我们对P450 2E1的预期完全一致。这些实验证据应能阐明大鼠脑中P450 2E1的存在。
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