Ritchie H, Wang L H
Department of Pediatrics, University of Iowa, Iowa City 52242, USA.
Biochem Biophys Res Commun. 1997 Feb 13;231(2):425-8. doi: 10.1006/bbrc.1997.6126.
We previously reported that the cDNA for dentin phosphophoryn, a non-collagenous protein involved in dentin mineralization, was located immediately downstream from the 3' end of dentin sialoprotein, another functionally related non-collagenous dentin protein (Ritchie, H. H., and Wang, L-H. (1996) J. Biol. Chem. 269, 3689-3702). We now demonstrate unequivocally that at the genomic level in mammal, these two functionally related proteins are indeed transcribed as one transcriptional unit suggesting that their combined presence at specific mineralization sites in tooth germ is mandatory if dentin mineralization is to proceed.
我们先前报道过，牙本质磷蛋白（一种参与牙本质矿化的非胶原蛋白）的cDNA位于牙本质涎蛋白（另一种功能相关的牙本质非胶原蛋白）3'端的紧邻下游（Ritchie, H. H., and Wang, L-H. (1996) J. Biol. Chem. 269, 3689 - 3702）。我们现在明确证明，在哺乳动物基因组水平上，这两种功能相关的蛋白质确实作为一个转录单元进行转录，这表明如果牙本质矿化要继续进行，它们在牙胚特定矿化位点的共同存在是必不可少的。
