Ligon A H, Pershouse M A, Jasser S A, Yung W K, Steck P A
Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston 77030, USA.
Oncogene. 1997 Mar 6;14(9):1075-81. doi: 10.1038/sj.onc.1200925.
Genetic deletions to chromosome 10 have been extensively documented for human glioblastomas (GBMs). To identify gene products that may be involved in malignant progression, a subtractive hybridization was performed between GBM cells and hybrid cells suppressed for tumorigenicity following microcell transfer of chromosome 10. One novel cDNA isolated from this subtraction showed consistent upregulation (approximately 4 to 10-fold) that correlated with the nontumorigenic phenotype of the hybrid cells. Subsequent analysis resulted in the identification of a full length cDNA (2,569 bp) termed RIG (regulated in glioma). RIG expression was either not detected or detected only at low levels in cultured glioma cells and primary glioblastoma specimens compared to normal brain cells. The 2.6 kb RIG mRNA was expressed predominantly in normal brain with lower levels in heart and lung. Sequence analysis showed no significant homology to known gene products. Genomic alterations of RIG were present in approximately 25% of glioma cell lines examined. Also, RIG mapped to chromosome 11p15.1, a region that is known to be altered in malignant astrocytomas. The differential expression pattern, tissue distribution and chromosomal location of RIG suggests it serves as a molecular marker for or may play a role in the malignant progression of GBMs.
人类胶质母细胞瘤（GBM）中10号染色体的基因缺失已有大量文献记载。为了鉴定可能参与恶性进展的基因产物，在GBM细胞与经10号染色体微细胞转移后致瘤性受到抑制的杂交细胞之间进行了消减杂交。从该消减杂交中分离出的一个新的cDNA显示出一致的上调（约4至10倍），这与杂交细胞的非致瘤表型相关。随后的分析鉴定出一个全长cDNA（2569 bp），称为RIG（胶质瘤中受调控）。与正常脑细胞相比，在培养的胶质瘤细胞和原发性胶质母细胞瘤标本中未检测到RIG表达或仅检测到低水平表达。2.6 kb的RIG mRNA主要在正常脑中表达，在心脏和肺中表达水平较低。序列分析表明与已知基因产物无明显同源性。在所检测的约25%的胶质瘤细胞系中存在RIG的基因组改变。此外，RIG定位于11p15.1染色体区域，该区域在恶性星形细胞瘤中已知会发生改变。RIG的差异表达模式、组织分布和染色体定位表明它可作为GBM恶性进展的分子标志物或可能在其中发挥作用。
