线粒体膜蛋白肉碱棕榈酰转移酶I与Bcl-2的直接相互作用。
Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2.
作者信息
Paumen M B, Ishida Y, Han H, Muramatsu M, Eguchi Y, Tsujimoto Y, Honjo T
机构信息
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
出版信息
Biochem Biophys Res Commun. 1997 Feb 24;231(3):523-5. doi: 10.1006/bbrc.1997.6089.
PMID:9070836
Abstract
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
摘要
为了证明线粒体膜蛋白CPT I和Bcl-2之间的相互作用，使用了酵母双杂交系统。与Bcl-2结合需要全长CPT I。在GST结合试验以及使用两种不同抗Bcl-2抗体的共免疫沉淀试验中证实了蛋白质之间的直接相互作用。
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