Paumen M B, Ishida Y, Han H, Muramatsu M, Eguchi Y, Tsujimoto Y, Honjo T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):523-5. doi: 10.1006/bbrc.1997.6089.
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
为了证明线粒体膜蛋白CPT I和Bcl-2之间的相互作用，使用了酵母双杂交系统。与Bcl-2结合需要全长CPT I。在GST结合试验以及使用两种不同抗Bcl-2抗体的共免疫沉淀试验中证实了蛋白质之间的直接相互作用。
