家蚕附着血细胞对大肠杆菌的体外吞噬作用及脂多糖释放
In vitro phagocytosis of Escherichia coli and release of lipopolysaccharide by adhering hemocytes of the silkworm, Bombyx mori.
作者信息
Taniai K, Wago H, Yamakawa M
机构信息
Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1997 Feb 24;231(3):623-7. doi: 10.1006/bbrc.1997.6151.
A primary culture containing adhering hemocytes mainly granular cells from the silkworm, Bombyx mori, was used to investigate in vitro phagocytosis of Escherichia coli. Phagocytosis was confirmed to occur in this system by microscopic observation. Lipopolysaccharide (LPS) concentration in the culture medium was measured by a Limulus test and a higher LPS concentration was detected in phagocytosis-occurred samples than in control samples, which omitted either E. coli cells or adhering hemocytes. Moreover, it was found that LPS containing sample but not control samples strongly induces gene expression of cecropin B, an antibacterial protein. These results suggest that bacterial cell wall components like LPS released by phagocytosis play an important role in the induction of insect antibacterial proteins.
利用一种原代培养物（主要包含来自家蚕的附着血细胞，即颗粒细胞）来研究大肠杆菌的体外吞噬作用。通过显微镜观察证实该系统中发生了吞噬作用。采用鲎试剂法测定培养基中的脂多糖（LPS）浓度，结果发现在发生吞噬作用的样本中检测到的LPS浓度高于对照样本，对照样本中要么不含大肠杆菌细胞，要么不含附着血细胞。此外，还发现含有LPS的样本而非对照样本能强烈诱导抗菌蛋白天蚕素B的基因表达。这些结果表明，吞噬作用释放的诸如LPS等细菌细胞壁成分在昆虫抗菌蛋白的诱导过程中发挥着重要作用。
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