Sarkar M, Chatterjee T
Indian Institute of Chemical Biology, Jadavpur, Calcutta, India.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):662-6. doi: 10.1006/bbrc.1997.6165.
The sperm surface antigens are organized into topographic domains, specifically formed into the membrane during the sperm maturation in the epididymis. The present study was directed to determine the distribution of 105 KD sperm membrane antigen (SMA2) into the cell by affinity purified antibody, generated by 105 KD sperm antigen. The cytoimmunofluorescence study of the integrated sperm cell reveal that the antigen is localized in the anterior head overlying the acrosome. On immunoblotting of isolated head and flagellum polypeptides it has been demonstrated that this antigen is selectively present in the head membrane and absent in the flagellum. Because of its selective presence in the anterior head membrane overlying the region of acrosome on mature spermatozoa, the 105 KD sperm unique glyco-antigen may have a role in events leading to sperm-egg recognition. It is a first report of a high molecular mass membrane antigen being localized in the anterior head membrane of goat mature epididymal spermatozoa.
精子表面抗原被组织成拓扑结构域，特别是在附睾中精子成熟过程中形成于细胞膜上。本研究旨在通过由105KD精子抗原产生的亲和纯化抗体来确定105KD精子膜抗原（SMA2）在细胞内的分布。对完整精子细胞的细胞免疫荧光研究表明，该抗原定位于覆盖顶体的头部前端。对分离的头部和鞭毛多肽进行免疫印迹分析表明，这种抗原选择性地存在于头部膜中，而不存在于鞭毛中。由于其在成熟精子顶体区域上方的头部前端膜中选择性存在，105KD精子独特的糖抗原可能在导致精卵识别的过程中发挥作用。这是关于高分子量膜抗原定位于山羊成熟附睾精子头部前端膜的首次报道。
