Yatomi Y, Ozaki Y, Satoh K, Kume S, Ruan F, Igarashi Y
Department of Laboratory Medicine, Yamanashi Medical University, Japan.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):848-51. doi: 10.1006/bbrc.1997.6207.
Metabolism of sphingosine (Sph) derivatives in human platelets was examined. [3H]Sph was rapidly and heavily phosphorylated into sphingosine 1-phosphate, similarly in resting and stimulated platelets. [14C]N,N-dimethylsphingosine was stable in resting platelets, while it was converted into N,N-dimethylsphingosine 1-phosphate (DMS-1-P), although weakly, in platelets stimulated with thrombin or 12-O-tetradecanoylphorbol 13-acetate. This DMS-1-P formation was inhibited by staurosporine, a potent protein kinase inhibitor. [3H]C2-ceramide was unchanged both in resting and stimulated platelets. Our report is the first to describe production of DMS-1-P in a biological system.
对人血小板中鞘氨醇（Sph）衍生物的代谢进行了研究。[3H]鞘氨醇在静息和受刺激的血小板中均迅速且大量磷酸化为1-磷酸鞘氨醇。[14C]N，N-二甲基鞘氨醇在静息血小板中稳定，但在用凝血酶或12-O-十四烷酰佛波醇-13-乙酸酯刺激的血小板中，虽转化较弱，但会转化为N，N-二甲基鞘氨醇1-磷酸（DMS-1-P）。这种DMS-1-P的形成受到强效蛋白激酶抑制剂星形孢菌素的抑制。[3H]C2-神经酰胺在静息和受刺激的血小板中均未发生变化。我们的报告首次描述了生物系统中DMS-1-P的产生。
